The effect of temperature on germination of chlamydospores of Phytophthora ramorum.
Identifieur interne : 000F49 ( Main/Exploration ); précédent : 000F48; suivant : 000F50The effect of temperature on germination of chlamydospores of Phytophthora ramorum.
Auteurs : Paul W. Tooley [États-Unis] ; Marsha Browning [États-Unis] ; Robert M. Leighty [États-Unis]Source :
- Mycologia [ 0027-5514 ]
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Phytophthora, Spores.
- microbiologie : Maladies des plantes.
- Facteurs temps, Température.
English descriptors
- KwdEn :
- MESH :
- growth & development : Phytophthora, Spores.
- microbiology : Plant Diseases.
- Temperature, Time Factors.
Abstract
Mycelium-free chlamydospores of 12 isolates of P. ramorum representing three clonal lineages were produced with a method involving incubation in nonsterile sand at 20 C in darkness for 30 d. Chlamydospores were incubated on selective agar medium at 5, 10, 15, 20, 25 and 30 C and germination assessed after 1, 2, 4, 6 and 8 d incubation. The optimal temperature for germination based on 8 d incubation was 20 C for all three clonal lineages tested (NA1, NA2, EU1). Mean germination rates were 2, 21, 44, 67, 32 and 0 percent at 5, 10, 15, 20, 25 and 30 C respectively for all isolates combined. The highest mean germination rate was scored by isolates of the EU1 clonal lineage at 20 C (85%) after 8 d incubation However, substantial variation was observed among isolates within each clonal lineage. Overall temperatures and days of incubation on which germination was assessed isolates of the NA1 clonal lineage had the lowest mean germination, even though one isolate had the highest germination of any isolate in any lineage. The results indicate that 20 C is the optimal germination temperature for P. ramorum chlamydospores and that a great disparity in germination percentage can exist within isolates, even within a single clonal lineage.
DOI: 10.3852/13-313
PubMed: 24871605
Affiliations:
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Leighty</name><affiliation wicri:level="2"><nlm:affiliation>Data Management Services Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Data Management Services Inc., Frederick National Laboratory for Cancer Research, Frederick</wicri:cityArea></affiliation></author></analytic><series><title level="j">Mycologia</title><idno type="ISSN">0027-5514</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Phytophthora (growth & development)</term><term>Plant Diseases (microbiology)</term><term>Spores (growth & development)</term><term>Temperature (MeSH)</term><term>Time Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Facteurs temps (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Phytophthora (croissance et développement)</term><term>Spores (croissance et développement)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Temperature</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Facteurs temps</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mycelium-free chlamydospores of 12 isolates of P. ramorum representing three clonal lineages were produced with a method involving incubation in nonsterile sand at 20 C in darkness for 30 d. Chlamydospores were incubated on selective agar medium at 5, 10, 15, 20, 25 and 30 C and germination assessed after 1, 2, 4, 6 and 8 d incubation. The optimal temperature for germination based on 8 d incubation was 20 C for all three clonal lineages tested (NA1, NA2, EU1). Mean germination rates were 2, 21, 44, 67, 32 and 0 percent at 5, 10, 15, 20, 25 and 30 C respectively for all isolates combined. The highest mean germination rate was scored by isolates of the EU1 clonal lineage at 20 C (85%) after 8 d incubation However, substantial variation was observed among isolates within each clonal lineage. Overall temperatures and days of incubation on which germination was assessed isolates of the NA1 clonal lineage had the lowest mean germination, even though one isolate had the highest germination of any isolate in any lineage. The results indicate that 20 C is the optimal germination temperature for P. ramorum chlamydospores and that a great disparity in germination percentage can exist within isolates, even within a single clonal lineage. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24871605</PMID><DateCompleted><Year>2014</Year><Month>09</Month><Day>04</Day></DateCompleted><DateRevised><Year>2014</Year><Month>06</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0027-5514</ISSN><JournalIssue CitedMedium="Print"><Volume>106</Volume><Issue>3</Issue><PubDate><MedlineDate>2014 May-Jun</MedlineDate></PubDate></JournalIssue><Title>Mycologia</Title><ISOAbbreviation>Mycologia</ISOAbbreviation></Journal><ArticleTitle>The effect of temperature on germination of chlamydospores of Phytophthora ramorum.</ArticleTitle><Pagination><MedlinePgn>424-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3852/13-313</ELocationID><Abstract><AbstractText>Mycelium-free chlamydospores of 12 isolates of P. ramorum representing three clonal lineages were produced with a method involving incubation in nonsterile sand at 20 C in darkness for 30 d. Chlamydospores were incubated on selective agar medium at 5, 10, 15, 20, 25 and 30 C and germination assessed after 1, 2, 4, 6 and 8 d incubation. The optimal temperature for germination based on 8 d incubation was 20 C for all three clonal lineages tested (NA1, NA2, EU1). Mean germination rates were 2, 21, 44, 67, 32 and 0 percent at 5, 10, 15, 20, 25 and 30 C respectively for all isolates combined. The highest mean germination rate was scored by isolates of the EU1 clonal lineage at 20 C (85%) after 8 d incubation However, substantial variation was observed among isolates within each clonal lineage. Overall temperatures and days of incubation on which germination was assessed isolates of the NA1 clonal lineage had the lowest mean germination, even though one isolate had the highest germination of any isolate in any lineage. The results indicate that 20 C is the optimal germination temperature for P. ramorum chlamydospores and that a great disparity in germination percentage can exist within isolates, even within a single clonal lineage. </AbstractText><CopyrightInformation>© 2014 by The Mycological Society of America.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tooley</LastName><ForeName>Paul W</ForeName><Initials>PW</Initials><AffiliationInfo><Affiliation>USDA-ARS Foreign Disease-Weed Science Research Unit, 1301 Ditto Avenue, Fort Detrick, Maryland 21702 paul.tooley@ars.usda.gov.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Browning</LastName><ForeName>Marsha</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>USDA-ARS Foreign Disease-Weed Science Research Unit, 1301 Ditto Avenue, Fort Detrick, Maryland 21702.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leighty</LastName><ForeName>Robert M</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>Data Management Services Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>05</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mycologia</MedlineTA><NlmUniqueID>0400764</NlmUniqueID><ISSNLinking>0027-5514</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013170" MajorTopicYN="N">Spores</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">overwintering</Keyword><Keyword MajorTopicYN="N">ramorum blight</Keyword><Keyword MajorTopicYN="N">resting spore</Keyword><Keyword MajorTopicYN="N">sudden oak death</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>5</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>5</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>9</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24871605</ArticleId><ArticleId IdType="pii">13-313</ArticleId><ArticleId IdType="doi">10.3852/13-313</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Tooley, Paul W" sort="Tooley, Paul W" uniqKey="Tooley P" first="Paul W" last="Tooley">Paul W. Tooley</name></region><name sortKey="Browning, Marsha" sort="Browning, Marsha" uniqKey="Browning M" first="Marsha" last="Browning">Marsha Browning</name><name sortKey="Leighty, Robert M" sort="Leighty, Robert M" uniqKey="Leighty R" first="Robert M" last="Leighty">Robert M. Leighty</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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